Tm difference between both primers, repeats and optimal primer-primer annealing Optimum parameters for PCR primer pairs.Optimum primer lengths, G/C as 3’-clamp, primer GC-content and primer Tm. In selecting appropriate primers, a variety of constraints on the primer and amplified product are already considered and are predefined as default values in our primer design tools: For sequencing primers, you can specify positions on the template that must be included in the sequencing. With Prime+ you can make use of one of th e best s oft ware analysing a DNA sequence to design PCR primer pairs or primers for sequencing.įor PCR primer pair selection, you can choose a target range of the template sequence to be amplified. Free design tools for perfect PCR and sequencing primersĮurofins Genomics' primer design tools are using Prime+ of the GCG Wisconsin Package originally written by Irv Edelman.
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